AR in Algiculture

Antimicrobials misuse in the livestock sector, aquaculture and crop production is a major concern as a risk for emergence and spread of antimicrobial resistant micro-organisms." The Food and Agriculture Organization of the United Nations

Agricultural food production is a hotspot for the dissemination of antibiotic resistance in the environment. Existing data suggests that antibiotic use in animals is as high, or in some countries even higher, as antibiotic use in humans. Antibiotic resistance can occur and develop in the gut microbiota of farmed animals and get released into the environment via animal waste. As most animal waste in agriculture is used as manure fertilizer for food production, there are concerns that antibiotic resistance may spread from Farm to Fork.

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AR in Hospitals

COVID-19 can create a perfect storm for antibiotic-resistant infections in healthcare settings” US Centers for Disease Control and Prevention

There are two important limitations to how antibiotic resistance is currently monitored in hospitals. First, current monitoring focuses mainly on a limited number of pathogenic bacteria. Second, it is often based on passive surveillance of bacteria isolated from patients. This leads to delayed detection of outbreaks, non-comparable data, and the inability to capture other pathogenic bacteria and antibiotic resistance profiles which are often carried by commensal bacteria.

Though not a substitute for existing monitoring methods, wastewater-based monitoring can provide data that is otherwise hard to obtain and become the easiest means for obtaining comprehensive information on the prevalence of resistance in hospitals. As waste from all patients is released into wastewater, wastewater monitoring can cover a wider range of antibiotic resistance profiles compared to the partial data from a few selected pathogenic bacteria. The practical, logistical and ethical barriers to sampling wastewater are also limited.

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AR in Water Environment

Antibiotic resistance is an emerging water issue

Antibiotic resistant bacteria and resistance genes contained in human and animal waste are released into surface waters from wastewater treatment plants, sewage overflows, and in run-off from agricultural fields fertilised with manure. These contaminated water environments may then act as exposure routes to humans and animals, particularly if the contaminated water is used for agricultural irrigation, drinking water production, or leisure activities such as swimming. Water environments may also facilitate the emergence and the spread of resistance to different places. We support the goals of the European Green Deal and seek to protect aquatic environments.

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ANTIBIOTIC RESISTANCE
GENE PROFILING SERVICE

Resistomap offers a complete laboratory and analysis service to monitor antibiotic
resistance genes in any sample type.

Combining multiwell high-throughput qPCR with automated raw data-analysis and interactive visualization creates a powerful platform for antibiotic resistance gene monitoring.

High-Throughtput Antibiotic Resistance Gene (ARG) Quantification

384 pre-validated ARG assays
SmartChip™ qPCR technology.

Automated
Data Analysis

Quantification relative to 16S rRNA gene. Interactive heatmap for ARG levels. Custom Analysis as service.

Sampling and
DNA Extraction

Sample collection advice and service.
DNA extraction from any sample

Antibiotic Resistance
Gene quantification
384 pre-validated ARG assays
SmartChip™ qPCR technology
Data Analysis
Quantification relative to 16S gene
Interactive heatmap for ARG levels
Custom analysis as a service
Sample Handling

Sample collection advice and service
DNA extraction from any sample

Sampling

We assist and give consultation on sampling best practices, (e.g. selecting the right DNA extraction kits), experimental design, and
routine monitoring planning.

Shipping

We recommend to shipping the samples using international courier service with tracking number.

SmartChip™ qPCR

SmartChip is a configurable multiwell real-time PCR platform. We have configured the SmartChip platform to fit your needs and samples: Exploratory screening with 384 genes, followed with customizable subset for focusing or monitoring most important hits, or for comprehensive profiling all 384 genes.

Delivery

Quantification of ARGs relative to 16S rRNA, presented in an interactive heatmap. Interpretation of results and custom figures are also available upon request.

Flexible Configuration

Flexibility of the SmartChip™ platform allows for high gene-content screening and high-throughput monitoring configurations

Genes
Samples
12
128
24
72
36
48
48
36
54
32
72
24
80
21
96
18
120
14
144
12
216
8
248
6
296
5
384
4
Genes
Samples
12
128
24
72
36
48
48
36
54
32
72
24
80
21
96
18
120
14
144
12
216
8
248
6
296
5
384
4

Prices

Prices without 24% VAT
Smartchip + analysis report
1 800 €
per chip
Smartchip only
1 200 €
per chip
Smartchip + analysis report
2 300 €
per chip

Service Fee

Prices exclude 24% VAT

Smartchip +
Data Analysis
1 800 €
per chip
Smartchip Only
1 200 €
per chip

Additional Service

DNA extraction


50 €
per sample
Extra Statistical
Analysis

starting
200 €
per analysis
Customized
SmartChip fee

starting
250 €
per project
Consultation


starting
250 €
per project

Service Fee

Prices exclude 24% VAT

Smartchip +
Data Analysis
1 800 €
per chip
Smartchip
only
1 200 €
per chip

Additional Service

DNA
extraction
50 €
per sample
Extra Statistical
Analysis
starting
200 €
per analysis
Customized
SmartChip fee
starting
250 €
per project
Consultation
starting
250 €
per project